NAV

The application of ion exchange resin in chromatographic separation is not only in the separation of inorganic compound, but also in the separation of organic acids such as sugars, proteins, amino acids, alkaline earth metals, heavy metals, rare earth metals, etc.
1、 The principle of resin chromatography separation: Ion exchange method is a method of separation that utilizes the exchange reaction between ions in ion exchange resin (solid phase) and solution (liquid phase). Mainly used for the enrichment of trace components and the preparation of high-purity substances. The affinity of resin for ions reflects the exchange ability of ions on the resin. The smaller the radius of hydrated ions, the higher the charge, and the greater their affinity. A solution containing different ions is passed through an ion exchange resin, and the resin will adsorb them onto the functional groups of the resin based on their different affinities to these ions (which is actually similar to the ion layer distribution diagram on water treatment). Then, different eluting solutions are selected to elute these adsorbents in sequence. Through the analysis of the eluting solution, the material components of different time periods are selected for recycling.
In the extraction and separation of organic substances, the main reliance is on physical factors such as resin polarity, pore size, specific surface area, and particle size. Different components in the organic mixture are sequentially adsorbed on the resin according to their molecular weight. After saturation, they are then analyzed and segmented for recovery and concentration through different solvents.
2、 The core component of chromatographic separation is the chromatographic column, and the quality of the filler (stationary phase) is the core of the chromatographic column. There are various types of fillers for chromatographic columns, including silicone, polymer resin, etc. Nowadays, more and more resins are used as fillers. The selection of fillers mainly depends on the separation mode of the sample.
3、 The performance of a chromatographic column is mainly determined by the following factors:
1. The quality issue of the filling resin itself mainly includes its physical and chemical performance indicators.
2. The aspect ratio of the chromatographic column. The higher the aspect ratio, the better the separation effect, but the resistance to operation is also relatively high. The ratio of height to diameter is better at 4:1.
3. The quality of the material is related. The temperature of the material solution has a certain relationship with the color scheme.
4. The selection of eluent and the concentration also have a certain impact on the elution effect. Therefore, it is necessary to conduct certain experiments to determine the optimal operating conditions for the chromatographic separation of a mixture.
4、 The chromatographic separation method of our DTF-01 chromatographic resin on oligosaccharide and glucose is introduced as follows:
DTF-01 chromatographic resin needs to undergo pre-treatment before use (if it is directly in the Rca type, it should be washed with pure water before use) to ensure that the resin reaches its normal state during use. The steps of preprocessing are:
1. After the resin is loaded into the column, it is soaked in pure water to make the resin fully absorb water and expand. Then, it is backwashed to control an appropriate expansion rate (80-100%) to remove fine resin particles and possible impurities until the backwashed water is clear, colorless and free of foam;
2. Inject 1mol/l of HCl solution, soak the resin for 24 hours when the outlet concentration reaches 0.5mol/l, and then rinse with pure water until neutral;
3. Inject approximately 0.5mol/l of NaOH solution, soak for 24 hours when the outlet concentration reaches 0.3 mol/l, and then rinse with pure water until neutral;
4. Transformation: Inject approximately 3BV-4BV of l mol/lCaCl2 solution to completely convert the resin to R2Ca type (if the factory is R2Ca type, only backwashing and cleaning are required), soak for more than 12 hours, and then wash with pure water to neutral for backup.
5、 Chromatographic separation operation: The material liquid at a certain temperature (70 ℃) passes through the resin column (or chromatographic column) at a certain flow rate. When the sugar liquid completely enters the chromatographic column and saturates the column (or there is a certain concentration of sugar at the outlet), the resin is eluted with pure water at the same flow rate, and the elution liquid is collected at the outlet of the resin column, and the composition and concentration of sugar are sampled and analyzed in a timely manner.
Separation effect: at 70 ℃, the elution flow rate is 5.6m/h, and the separation effect of DTF-01 resin is that the content of oligosaccharide is as high as 92%, while the content of glucose is only 1.6% at the beginning of the collection of separation solution. With the passage of time, the outflow of oligosaccharide gradually decreased, and the concentration changed rapidly.
The test results showed that the separation and purification of oligosaccharide produced by fermentation with our DTF-01 resin could achieve good results. Most glucose components in oligosaccharide can be removed, so that the content of oligosaccharide increases from 60% to more than 90%, and the content of glucose is less than 2%.
Similarly, DTF series chromatography separation resins have mature and good separation effects for glucose and fructose separation, as well as for the purification and separation of Chinese herbal medicine. In addition, the same resin exhibits different ionic forms and its functions also differ, such as:
Calcium type: fructose/glucose; Sorbitol/Mannitol; Mannose/dextrose; Galactose/glucose
Sodium or potassium type: dextrose/polymer; Maltose/polymer;